利用某些微生物代謝過(guò)程中產(chǎn)生酶的特點(diǎn),篩選出能夠大量生成葡萄糖氧化酶的地衣芽孢桿菌,并將其固定在Sephadex 100 或海藻酸納- 氯化鈣(載體) 上,首次研制成微生物酶傳感器. 它不僅具有酶?jìng)鞲衅鞯乃袃?yōu)點(diǎn),同時(shí)也克服了酶?jìng)鞲衅鞯娜秉c(diǎn),使用壽命長(zhǎng),性質(zhì)穩(wěn)定,成本低,易于保存等. 通過(guò)優(yōu)化微生物和其代謝所產(chǎn)生的葡萄糖氧化酶的固定化方法和利用葡萄糖氧化酶氧化葡萄糖產(chǎn)生過(guò)氧化氫的反應(yīng)機(jī)理,建立了模擬酶(hemin) 催化熒光底
物N , N′2 二腈甲基鄰苯二氨(DCM2OPA) 檢測(cè)體液中葡萄糖含量的熒光傳感器.
關(guān)鍵詞　微生物傳感器,酶?jìng)鞲衅?熒光,葡萄糖
Abstract 　A microbial based biosensor for the measurement of glucose in serum and urine has been developed. Bscillus licheniformis , a microbial , was used in the cytosensor as the producer of glucose oxidase that are required in the biosensor. The influence of the types of microbes , enzyme immobilizing techniques and the carrier for the enzyme or microbial were studied. The new sensor has the advantages of high stability , low cost and easier preservation. It is the first time to develop a cytosensor with a mimic enzyme catalyzed fluorescence reaction for the measurement of glucose in serum and urine.
Keywords 　microbial sensor , enzyme sensor , fluorescence , chemiluminescence , glucose